Product Info Summary
SKU: | A03971 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ICC, WB |
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Product info
Product Name
Anti-UBE2D2 Antibody
View all UbcH5b/UBE2D2 Antibodies
SKU/Catalog Number
A03971
Size
100ul
Form
Liquid
Description
Boster Bio Anti-UBE2D2 Antibody catalog # A03971. Tested in WB,ICC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE2D2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03971)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human WASF2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03971 is reactive to UBE2D2 in Human, Mouse, Rat
Applications
A03971 is guaranteed for ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
16.735kDa
Background of UbcH5b/UBE2D2
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Suetsugu S., Biochem. Biophys. Res. Commun. 260:296-302(1999).
Gregory S.G., Nature 441:315-321(2006).
Miki H., Nature 408:732-735(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
ICC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-UBE2D2 Antibody A03971
Western blot (WB) analysis of UBE2D2 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysate
Lane2:Raw264.7 cell lysate
Lane3:H9C2 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For UBE2D2 (Source: Uniprot.org, NCBI)
Gene Name
UBE2D2
Full Name
Ubiquitin-conjugating enzyme E2 D2
Weight
16.735kDa
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
E2(17)KB2; EC 6.3.2.19; PUBC1; UBC4/5; UBC4ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); UBC5B; UBCH4; UbcH5b; UBE2D2; Ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2 D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D2 UBE2D2 E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B ubiquitin conjugating enzyme E2 D2 ubiquitin-conjugating enzyme E2 D2|(E3-independent) E2 ubiquitin-conjugating enzyme D2|E2 ubiquitin-conjugating enzyme D2|p53-regulated ubiquitin-conjugating enzyme 1|ubiquitin carrier protein D2|ubiquitin conjugating enzyme E2D 2|ubiquitin-conjugating enzyme E2-17 kDa 2|ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)|ubiquitin-protein ligase D2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBE2D2, check out the UBE2D2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBE2D2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBE2D2 Antibody (A03971)
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No publications found for A03971
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